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pME-APEX2-mCherry
(Plasmid #188944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Kan
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 2579
  • Total vector size (bp) 5681
  • Vector type
    Middle Entry vector for Gateway (L1-L2)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mito-APEX2_p2A_APEX2-H2B_p2A_mCherry
  • Alt name
    APEX2-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    3102

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-APEX2-mCherry was a gift from Owen Tamplin (Addgene plasmid # 188944 ; http://n2t.net/addgene:188944 ; RRID:Addgene_188944)

  • For your References section:

    Defining the ultrastructure of the hematopoietic stem cell niche by correlative light and electron microscopy. Agarwala S, Kim KY, Phan S, Ju S, Kong YE, Castillon GA, Bushong EA, Ellisman MH, Tamplin OJ. Elife. 2022 Aug 9;11. pii: 64835. doi: 10.7554/eLife.64835. 10.7554/eLife.64835 PubMed 35943143
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