pGEX-Ubc13
(Plasmid #18894)

• Depositing Lab: Cecile Pickart
• Publication: Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Backbone

• Vector backbone: pGEX4T2
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin conjugating enzyme UBC13
• Alt name: UBC13
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: UBC13 (a.k.a. YDR092W)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: 5'-pGEX primer
• 3′ sequencing primer: 3'-pGEX primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cecile Pickart
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry