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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET16b
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsBL21pJY2
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameYeast ubiquitin conjugating enzyme Mms2
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneMMS2 (a.k.a. YGL087C)
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Tag
/ Fusion Protein
- HIS10 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid is constructed by Hofmann R.M.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Hofmann R.M. Pickart C.M. 1999, Cell 96, 645-653
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET16b-Mms2 was a gift from Cecile Pickart (Addgene plasmid # 18893 ; http://n2t.net/addgene:18893 ; RRID:Addgene_18893) -
For your References section:
Ubiquitin chain synthesis. Raasi S, Pickart CM. Methods Mol Biol. 2005 . 301():47-55. 10.1385/1-59259-895-1:047 PubMed 15917625