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Addgene

pBS-SKII-3XHA-eDHFR-Hygromycin
(Plasmid #188924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK (+)
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3XHA-eDHFR-Hygromycin
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-SKII-3XHA-eDHFR-Hygromycin was a gift from Stephen Buratowski (Addgene plasmid # 188924 ; http://n2t.net/addgene:188924 ; RRID:Addgene_188924)

  • For your References section:

    A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins. Baek I, Le SN, Jeon J, Chun Y, Reed C, Buratowski S. G3 (Bethesda). 2022 Aug 9. pii: 6659097. doi: 10.1093/g3journal/jkac201. 10.1093/g3journal/jkac201 PubMed 35944214
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