pET3d-E2-25K-C170S
(Plasmid #18892)

• Depositing Lab: Cecile Pickart
• Publication: Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Backbone

• Vector backbone: pET3d
• Backbone size w/o insert (bp): 4600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubiquitin conjugating enzyme E2-25K
• Alt name: E2-25K
• Species: H. sapiens (human); Most likely H. sapiens
• Mutation: Cysteine 170 is replaced with a Serine residue.
• Entrez Gene: UBE2K (a.k.a. E2-25K, HIP2, HYPG, LIG, UBC1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N7A (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cecile Pickart
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry