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Addgene

pEF mito-PAcGFP
(Plasmid #188905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitochondrial photoactivatable GFP
  • Species
    Synthetic
  • Tag / Fusion Protein
    • Mitochondrial leader sequence: MSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPPVAT (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.07.06.499030 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF mito-PAcGFP was a gift from Edwin Chapman (Addgene plasmid # 188905 ; http://n2t.net/addgene:188905 ; RRID:Addgene_188905)

  • For your References section:

    Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca(2+) Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons. Vevea JD, Chapman ER. J Neurosci. 2023 May 10;43(19):3421-3438. doi: 10.1523/JNEUROSCI.1356-22.2023. Epub 2023 Mar 30. 10.1523/JNEUROSCI.1356-22.2023 PubMed 36997314
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