-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCMV-neo-Bam
- Backbone size w/o insert (bp) 6550
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCdc2
-
Alt namecell division cycle 2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)945
-
MutationD146N, dominant negative
-
Entrez GeneCDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
-
Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Bglob-intron-F
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See scanned map.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Cdc2-DN-HA was a gift from Sander van den Heuvel (Addgene plasmid # 1889 ; http://n2t.net/addgene:1889 ; RRID:Addgene_1889) -
For your References section:
Distinct roles for cyclin-dependent kinases in cell cycle control. van den Heuvel S, Harlow E. Science 1993 Dec 24;262(5142):2050-4. 10.1126/science.8266103 PubMed 8266103
Map uploaded by the depositor.
