pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
(Plasmid #188899)

• Purpose: dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
• Depositing Labs: Marco Jost, Jonathan Weissman
• Publication: Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.

Backbone

• Vector backbone: pMH0006, Addgene # 135448
• Vector type: Lentiviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Zim3-dCas9
• Species: Multiple
• Promoter: SFFV
• Tags / Fusion Proteins:
  • Zim3 KRAB-NLS fusion (N terminal on insert)
  • HA-2xNLS (C terminal on insert)
  • P2A-GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element. pJB69.

Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.