pLX-AS
(Plasmid
#188870)
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PurposeCompact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLX-B2
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Backbone manufacturerFabio Pasin
- Total vector size (bp) 4524
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Vector typePlant Expression, Synthetic Biology ; Binary T-DNA vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
- Promoter cauliflower mosaic virus (CaMV) 35S promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX-AS was a gift from Fabio Pasin (Addgene plasmid # 188870 ; http://n2t.net/addgene:188870 ; RRID:Addgene_188870) -
For your References section:
Assembly of plant virus agroinfectious clones using biological material or DNA synthesis. Pasin F. STAR Protoc. 2022 Dec 16;3(4):101716. doi: 10.1016/j.xpro.2022.101716. Epub 2022 Sep 22. 10.1016/j.xpro.2022.101716 PubMed 36149792
