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Addgene

PYBS375
(Plasmid #18887)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYBS317
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ste11
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1239
  • Mutation
    Lex-Ste11C (amino-terminal 413 amino acids)
  • Entrez Gene
    STE11 (a.k.a. YLR362W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PflMI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer pHybLex_fwd
  • 3′ sequencing primer pHybLex-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lex-Ste11 has the 0.9kb PflMI-SalI fragment of pYBS345 (the PCR-derived Ste11 in pYBS317; see article) deleted by blunt-end ligation with Klenow. The open reading frame reads through to an unknown stop in the vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PYBS375 was a gift from Elaine Elion (Addgene plasmid # 18887 ; http://n2t.net/addgene:18887 ; RRID:Addgene_18887)

  • For your References section:

    Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Choi KY, Satterberg B, Lyons DM, Elion EA. Cell. 1994 Aug 12. 78(3):499-512. 10.1016/0092-8674(94)90427-8 PubMed 8062390
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