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Addgene

pYBS370
(Plasmid #18886)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18886 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYBS311
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ste7
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1032
  • Mutation
    kinase domain of C-terminal 344 residues
  • Entrez Gene
    STE7 (a.k.a. YDL159W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer pHybLex_fwd
  • 3′ sequencing primer pHybLex-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lex-Ste7C has the 1 kb BglII-BamHI fragment of pYEE129 at BamHI of pYBS311

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYBS370 was a gift from Elaine Elion (Addgene plasmid # 18886 ; http://n2t.net/addgene:18886 ; RRID:Addgene_18886)

  • For your References section:

    Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Choi KY, Satterberg B, Lyons DM, Elion EA. Cell. 1994 Aug 12. 78(3):499-512. 10.1016/0092-8674(94)90427-8 PubMed 8062390
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