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Addgene

pYBS369
(Plasmid #18885)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYBS317
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ste7
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    516
  • Mutation
    amino terminal 172 aa of Ste7
  • Entrez Gene
    STE7 (a.k.a. YDL159W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer pHybLex_fwd
  • 3′ sequencing primer pHybLex-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Has a 0.5kb BamHI-BglII fragmetn from pYEE129 at BamHI of pYBS317. The open reading frame reads through to an unknown stop in the vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYBS369 was a gift from Elaine Elion (Addgene plasmid # 18885 ; http://n2t.net/addgene:18885 ; RRID:Addgene_18885)

  • For your References section:

    Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Choi KY, Satterberg B, Lyons DM, Elion EA. Cell. 1994 Aug 12. 78(3):499-512. 10.1016/0092-8674(94)90427-8 PubMed 8062390
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