pCDF-CcAvs4
(Plasmid
#188808)
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PurposeCodon optimized CcAvs4 with C-terminal His tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDF-1b
- Backbone size w/o insert (bp) 3423
- Total vector size (bp) 8733
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameKpAvs4
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SpeciesCorallococcus coralloides DSM2259
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Insert Size (bp)5310
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GenBank IDWP_083892326.1
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF-CcAvs4 was a gift from Feng Zhang (Addgene plasmid # 188808 ; http://n2t.net/addgene:188808 ; RRID:Addgene_188808) -
For your References section:
Prokaryotic innate immunity through pattern recognition of conserved viral proteins. Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2022 Aug 12;377(6607):eabm4096. doi: 10.1126/science.abm4096. Epub 2022 Aug 12. 10.1126/science.abm4096 PubMed 35951700
