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pBMN mEGFP-OPTN
(Plasmid #188784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN
  • Backbone size w/o insert (bp) 5750
  • Total vector size (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPTN
  • Alt name
    ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1730
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN mEGFP-OPTN was a gift from Michael Lazarou (Addgene plasmid # 188784 ; http://n2t.net/addgene:188784 ; RRID:Addgene_188784)

  • For your References section:

    Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M. Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. 10.1016/j.molcel.2023.04.021 PubMed 37207627
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