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pEXP(FLAG/HA-NSP1-K164-H165A)
(Plasmid #188782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Modifications to backbone
    Quite a few
  • Vector type
    Mammalian Expression ; FRT site to insert into HEK293 T-REx genome
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG/HA-NSP1-K164-H165A (SARS-CoV-2)
  • Alt name
    NSP1-K164-H165A
  • Species
    SARS-CoV-2
  • Mutation
    Changed Lysine 164 to Alanine and Histidine 165 to Alanine
  • Promoter CMV Dox inducible
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXP(FLAG/HA-NSP1-K164-H165A) was a gift from Markus Hafner (Addgene plasmid # 188782 ; http://n2t.net/addgene:188782 ; RRID:Addgene_188782)

  • For your References section:

    Epigenetic repression of antiviral genes by SARS-CoV-2 NSP1. Anastasakis DG, Benhalevy D, Cuburu N, Altan-Bonnet N, Hafner M. PLoS One. 2024 Jan 26;19(1):e0297262. doi: 10.1371/journal.pone.0297262. eCollection 2024. 10.1371/journal.pone.0297262 PubMed 38277395
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