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pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry
(Plasmid #188779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH0001, Addgene # 85969
  • Vector type
    Lentiviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zim3-dCas9
  • Species
    Multiple
  • Promoter SFFV
  • Tag / Fusion Protein
    • Zim3 KRAB-NLS fusion (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and EGFP linked by a P2A site from an EF1a promoter with an upstream ubiquitous chromatin opening element. pNM1123.

Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry was a gift from Marco Jost & Jonathan Weissman (Addgene plasmid # 188779 ; http://n2t.net/addgene:188779 ; RRID:Addgene_188779)

  • For your References section:

    Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M. eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856. 10.7554/eLife.81856 PubMed 36576240
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