pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
(Plasmid #188777)

• Purpose: dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS
• Depositing Labs: Marco Jost, Jonathan Weissman
• Publication: Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.

Backbone

• Vector backbone: pMH0006, Addgene # 135448
• Vector type: Lentiviral
• Selectable markers: mTagBFP2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Zim3-dCas9
• Species: Multiple
• Promoter: EF1a
• Tags / Fusion Proteins:
  • Zim3 KRAB-NLS fusion (N terminal on insert)
  • HA-2xNLS (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and mTagBFP2 linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element, pNM1125.

Please note that the Addgene NGS result differs from the depositor reference sequence under the Supplemental Documents section. These differences are minor and do not affect the plasmid's function. Please refer to the Addgene NGS result for the most accurate sequence information.

Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.