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PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE
(Plasmid #188759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJT126
  • Backbone manufacturer
    Michael Bassik, Lacra Bintu, Addgene #161926
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF473 KRAB
  • Species
    H. sapiens (human)
  • Entrez Gene
    ZNF473 (a.k.a. ZFP100, ZN473)
  • Promoter pEF1a
  • Tag / Fusion Protein
    • rTetR(SE-G72P)-3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 188759 ; http://n2t.net/addgene:188759 ; RRID:Addgene_188759)

  • For your References section:

    Development of compact transcriptional effectors using high-throughput measurements in diverse contexts. Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.. bioRxiv. (PrePrint) 10.1101/2023.05.12.540558
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