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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVAP-A
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Alt nameVAPA
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Alt nameVAP-33
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SpeciesR. norvegicus (rat)
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Insert Size (bp)726
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Mutationdouble point mutant K87D / M89D
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Entrez GeneVapa
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pCMV5F
- 3′ sequencing primer GFP-reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-VAPA(1-242) K87D/M89D was a gift from Axel Brunger (Addgene plasmid # 18875 ; http://n2t.net/addgene:18875 ; RRID:Addgene_18875) -
For your References section:
Structural basis of FFAT motif-mediated ER targeting. Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT. Structure. 2005 Jul . 13(7):1035-45. 10.1016/j.str.2005.04.010 PubMed 16004875