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Addgene

pMV2 AAV-RapaInducible-NFZ-HGF
(Plasmid #188749)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188749 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJT360
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HGF
  • Species
    H. sapiens (human)
  • Entrez Gene
    HGF (a.k.a. DFNB39, F-TCF, HGFB, HPTA, SF)
  • Promoter Rapamycin inducible

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector has small deletion in the 5' ITR region; not recommended for AAV production until corrected

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMV2 AAV-RapaInducible-NFZ-HGF was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 188749 ; http://n2t.net/addgene:188749 ; RRID:Addgene_188749)
  • For your References section:

    Development of compact transcriptional effectors using high-throughput measurements in diverse contexts. Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.. bioRxiv. (PrePrint) 10.1101/2023.05.12.540558