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Addgene

p2151 pHAGE-hSPC-TFEB-TagBFP-W
(Plasmid #188722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-EF1aL-eGFP-W
  • Total vector size (bp) 12044
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TFEB
  • Alt name
    transcription factor EB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • Entrez Gene
    TFEB (a.k.a. ALPHATFEB, BHLHE35, TCFEB)
  • Promoter hSP-C
  • Tag / Fusion Protein
    • tagBFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer cctggacgactcactgctac
  • 3′ sequencing primer aaatttGCGGCCGCgaccggtggatcccgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2151 pHAGE-hSPC-TFEB-TagBFP-W was a gift from Darrell Kotton (Addgene plasmid # 188722 ; http://n2t.net/addgene:188722 ; RRID:Addgene_188722)
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