phU6 ST
(Plasmid
#188712)
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PurposesgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonephU6
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Vector typeCRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSafe Targeting sgRNA
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gRNA/shRNA sequenceGATGGTGACAGTTGTCGA
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phU6 ST was a gift from David Tobin (Addgene plasmid # 188712 ; http://n2t.net/addgene:188712 ; RRID:Addgene_188712) -
For your References section:
Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection. Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM. Cell Rep. 2022 Dec 13;41(11):111817. doi: 10.1016/j.celrep.2022.111817. 10.1016/j.celrep.2022.111817 PubMed 36516756
