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Addgene

pmU6 NFATC2
(Plasmid #188709)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmU6
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFATC2 sgRNA
  • gRNA/shRNA sequence
    GCTTGGCACCAGGCGATG
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmU6 NFATC2 was a gift from David Tobin (Addgene plasmid # 188709 ; http://n2t.net/addgene:188709 ; RRID:Addgene_188709)

  • For your References section:

    Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection. Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM. Cell Rep. 2022 Dec 13;41(11):111817. doi: 10.1016/j.celrep.2022.111817. 10.1016/j.celrep.2022.111817 PubMed 36516756
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