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Addgene

pLV hUbC-Cas9-P2A-Puro_ST-sgRNA
(Plasmid #188705)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188705 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ST sgRNAs
  • gRNA/shRNA sequence
    GATGGTGACAGTTGTCGA, GCTAAGTACTCTAACAGG, GTGGATAACTTCCTGAGT, GTGCAGTTCTCCGGGTTG
  • Species
    Synthetic
  • Promoter hUbC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp3I (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV hUbC-Cas9-P2A-Puro_ST-sgRNA was a gift from David Tobin (Addgene plasmid # 188705 ; http://n2t.net/addgene:188705 ; RRID:Addgene_188705)

  • For your References section:

    Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection. Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM. Cell Rep. 2022 Dec 13;41(11):111817. doi: 10.1016/j.celrep.2022.111817. 10.1016/j.celrep.2022.111817 PubMed 36516756
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