pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
(Plasmid
#188704)
-
PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneFUGW
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNFATC2 sgRNAs
-
gRNA/shRNA sequenceGACACCGGCGAGGGGTCA, GCTTGGCACCAGGCGATG, GCCACGGACTCGCCTTGT, GGCCGGGTAGATGTGGCG
-
SpeciesSynthetic
- Promoter hUbC
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Esp3I (destroyed during cloning)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA was a gift from David Tobin (Addgene plasmid # 188704 ; http://n2t.net/addgene:188704 ; RRID:Addgene_188704) -
For your References section:
Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection. Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM. Cell Rep. 2022 Dec 13;41(11):111817. doi: 10.1016/j.celrep.2022.111817. 10.1016/j.celrep.2022.111817 PubMed 36516756
