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Addgene

p3e UbB polyA
(Plasmid #188702)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188702 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR p2r-p3
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbB polyA
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    235
  • Entrez Gene
    ubb (a.k.a. Ubi-p63E, im:6892314, si:ch211-202a12.3, ubc, zUBC, zgc:172187)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3e UbB polyA was a gift from David Tobin (Addgene plasmid # 188702 ; http://n2t.net/addgene:188702 ; RRID:Addgene_188702)
  • For your References section:

    The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish. Walton EM, Cronan MR, Beerman RW, Tobin DM. PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015. PONE-D-15-31888 [pii] PubMed 26445458