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Addgene

N-Cadherin-EGFP
(Plasmid #18870)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18870 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1 (Clontech)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-Cadherin
  • Alt name
    N-Cadherin EGFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • GenBank ID
    AB008811
  • Entrez Gene
    Cdh2 (a.k.a. CDHN, N-CAD, Ncad)
  • Tags / Fusion Proteins
    • EGFP (C terminal on insert)
    • N-Cadherin (N terminal on insert)
    • Kanamycin (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N-Cadherin-EGFP was a gift from Valeri Vasioukhin (Addgene plasmid # 18870 ; http://n2t.net/addgene:18870 ; RRID:Addgene_18870)
  • For your References section:

    Failure of epithelial tube maintenance causes hydrocephalus and renal cysts in Dlg5-/- mice. Nechiporuk T, Fernandez TE, Vasioukhin V. Dev Cell. 2007 Sep . 13(3):338-50. 10.1016/j.devcel.2007.07.017 PubMed 17765678