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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1 (Clontech)
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameN-Cadherin
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Alt nameN-Cadherin EGFP
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SpeciesM. musculus (mouse)
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Insert Size (bp)2700
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GenBank IDAB008811
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Entrez GeneCdh2 (a.k.a. CDHN, N-CAD, Ncad)
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Tags
/ Fusion Proteins
- EGFP (C terminal on insert)
- N-Cadherin (N terminal on insert)
- Kanamycin (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
N-Cadherin-EGFP was a gift from Valeri Vasioukhin (Addgene plasmid # 18870 ; http://n2t.net/addgene:18870 ; RRID:Addgene_18870) -
For your References section:
Failure of epithelial tube maintenance causes hydrocephalus and renal cysts in Dlg5-/- mice. Nechiporuk T, Fernandez TE, Vasioukhin V. Dev Cell. 2007 Sep . 13(3):338-50. 10.1016/j.devcel.2007.07.017 PubMed 17765678