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Addgene

SGEP-sh-Hs-PHGDH-1957
(Plasmid #188674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shPHGDH
  • gRNA/shRNA sequence
    TGCTGTTGACAGTGAGCGCGACCCTGTAGTACAGCAATAATAGTGAAGCCACAGATGTATTATTGCTGTACTACAGGGTCATGCCTACTGCCTCGGA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    97
  • Mutation
    WT
  • Entrez Gene
    PHGDH (a.k.a. 3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SGEP-sh-Hs-PHGDH-1957 was a gift from Lewis Cantley (Addgene plasmid # 188674 ; http://n2t.net/addgene:188674 ; RRID:Addgene_188674)
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