meGFP-hTRAK1
(Plasmid
#188664)
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Purposehuman TRAK1 with N-terminal meGFP tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188664 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneplasmid 54759
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Backbone manufactureraddgene
- Backbone size w/o insert (bp) 4750
- Total vector size (bp) 7569
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOIP106
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Alt nameMilton1, TRAK1
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SpeciesH. sapiens (human)
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Entrez GeneTRAK1 (a.k.a. DEE68, EIEE68, MILT1, OIP106)
- Promoter CMV
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Tag
/ Fusion Protein
- meGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BAMHI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGift from Gerald W. Hart (University of Georgia)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
meGFP-hTRAK1 was a gift from Thomas Schwarz (Addgene plasmid # 188664 ; http://n2t.net/addgene:188664 ; RRID:Addgene_188664)