AAV_A2S-8
(Plasmid #188654)

• Purpose: AAV vector expressing SaAID-2S and gRNA
• Depositing Lab: Keiji Nishida
• Publication: Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi: 10.1038/s41467-022-32157-8.

Backbone

• Vector backbone: pAAV2
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: AID2S--nSaCas9-UGI
• Species: Synthetic; Staphylococcus aureus
• Insert Size (bp): 5175
• Mutation: D10A for SaCas9
• GenBank ID:
• Promoter: Scp1
• Tag / Fusion Protein:
  • UGI

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gtacttatataagggggtgg
• 3′ sequencing primer: cacacaaaaaaccaacacac

Gene/Insert 2

• Gene/Insert name: Sa-sgRNA
• Species: Staphylococcus aureus
• Promoter: U6

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cagcctcgaaataaaagatc
• 3′ sequencing primer: gagggcctatttcccatg

Resource Information

• Supplemental Documents:
  • AAV_A2S-8.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry