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Addgene

Mammalian_SpCas9_Target-AID2S
(Plasmid #188648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nCas9-AID2S-UGI
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    5175
  • Mutation
    D10A for SpCas9
  • GenBank ID
    OYN72315.1 ABO15149.1
  • Promoter pCMV
  • Tag / Fusion Protein
    • UGI

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGCACCAAAATCAACGGGA
  • 3′ sequencing primer GCCCCAGCTGGTTCTTTCCGCCTCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mammalian_SpCas9_Target-AID2S was a gift from Keiji Nishida (Addgene plasmid # 188648 ; http://n2t.net/addgene:188648 ; RRID:Addgene_188648)

  • For your References section:

    Cytosine base editing systems with minimized off-target effect and molecular size. Li A, Mitsunobu H, Yoshioka S, Suzuki T, Kondo A, Nishida K. Nat Commun. 2022 Aug 8;13(1):4531. doi: 10.1038/s41467-022-32157-8. 10.1038/s41467-022-32157-8 PubMed 35941130
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