pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A
(Plasmid #18864)

• Depositing Lab: Tobias Meyer
• Publication: Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Backbone

• Vector backbone: Gateway destination vector
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Stromal interaction molecule 2
• Alt name: STIM2
• Species: H. sapiens (human)
• Mutation: EF hand mutant: D80A. YFP inserted between signal peptide(SP) of STIM1 and residues 15-746 of STIM2 (Lab plasmid WS5183)
• Entrez Gene: STIM2
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.