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Addgene

pTA-Mob 2.0 Tp
(Plasmid #188619)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTA-Mob
  • Backbone manufacturer
    Strand et al., 2014
  • Backbone size (bp) 56570
  • Modifications to backbone
    Insertion of yeast artificial chromosome elements (HIS3-CEN6-ARSH4 and URA3) Cluster mutation in TraJ promoter region
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology ; Conjugation helper plasmid
  • Selectable markers
    URA3, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300 (Epicentre)
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA-Mob 2.0 Tp was a gift from Bogumil Karas (Addgene plasmid # 188619 ; http://n2t.net/addgene:188619 ; RRID:Addgene_188619)

  • For your References section:

    Superior Conjugative Plasmids Delivered by Bacteria to Diverse Fungi. Cochrane RR, Shrestha A, Severo de Almeida MM, Agyare-Tabbi M, Brumwell SL, Hamadache S, Meaney JS, Nucifora DP, Say HH, Sharma J, Soltysiak MPM, Tong C, Van Belois K, Walker EJL, Lachance MA, Gloor GB, Edgell DR, Shapiro RS, Karas BJ. Biodes Res. 2022 Aug 19;2022:9802168. doi: 10.34133/2022/9802168. eCollection 2022. 10.34133/2022/9802168 PubMed 37850145
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