M8C8
(Plasmid
#188617)
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Purpose(Empty Backbone) Minimal Conjugative Plasmid 8
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTA-Mob 2.0
- Backbone size (bp) 57000
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Modifications to backboneDeletion: pTA-Mob 2.0 ΔTrbM-TraE, ΔKlaC-KleA, ΔTrfA-TraJ† Plasmid Size: 31319 bp
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300 (Epicentre)
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M8C8 was a gift from Bogumil Karas (Addgene plasmid # 188617 ; http://n2t.net/addgene:188617 ; RRID:Addgene_188617) -
For your References section:
Superior Conjugative Plasmids Delivered by Bacteria to Diverse Fungi. Cochrane RR, Shrestha A, Severo de Almeida MM, Agyare-Tabbi M, Brumwell SL, Hamadache S, Meaney JS, Nucifora DP, Say HH, Sharma J, Soltysiak MPM, Tong C, Van Belois K, Walker EJL, Lachance MA, Gloor GB, Edgell DR, Shapiro RS, Karas BJ. Biodes Res. 2022 Aug 19;2022:9802168. doi: 10.34133/2022/9802168. eCollection 2022. 10.34133/2022/9802168 PubMed 37850145