pEX-YFP-STIM1-deltaK
(Plasmid #18861)

• Depositing Lab: Tobias Meyer
• Publication: Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 104(22):9301-6.

Backbone

• Vector backbone: Gateway
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STIM1
• Species: H. sapiens (human)
• Mutation: Stop codon inserted at amino acid postion 671 of STIM1. YFP inserted between signal peptide (STIM1 aminoacids 1-23) and residues 23-670 of STIM1. (Lab plasmid TI1020)
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry