pEX-CFP-STIM1-deltaK
(Plasmid
#18860)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGateway
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTIM1
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SpeciesH. sapiens (human)
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MutationStop codon at amino acid position 671 of STIM1. CFP inserted between signal peptide (STIM1 aminoacids 1-23) and residues 23-670 of STIM1. (Lab plasmid TI1019)
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Entrez GeneSTIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
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Tag
/ Fusion Protein
- CFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attB1 (unknown if destroyed)
- 3′ cloning site attB2 (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEX-CFP-STIM1-deltaK was a gift from Tobias Meyer (Addgene plasmid # 18860 ; http://n2t.net/addgene:18860 ; RRID:Addgene_18860) -
For your References section:
Live-cell imaging reveals sequential oligomerization and local plasma membrane targeting of stromal interaction molecule 1 after Ca2+ store depletion. Liou J, Fivaz M, Inoue T, Meyer T. Proc Natl Acad Sci U S A. 2007 May 29. 104(22):9301-6. 10.1073/pnas.0702866104 PubMed 17517596
