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pAFMW-Nbr[K284A]
(Plasmid #188595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAFMW
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nibbler
  • Species
    D. melanogaster (fly)
  • Mutation
    K284A [AAG=> GCG]
  • Entrez Gene
    Nbr (a.k.a. Dmel_CG9247, CG9247, Dmel\CG9247, nbr)
  • Tag / Fusion Protein
    • FLAG-Myc (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAFMW-Nbr[K284A] was a gift from Stefan Ameres (Addgene plasmid # 188595 ; http://n2t.net/addgene:188595 ; RRID:Addgene_188595)

  • For your References section:

    Structure-function analysis of microRNA 3'-end trimming by Nibbler. Xie W, Sowemimo I, Hayashi R, Wang J, Burkard TR, Brennecke J, Ameres SL, Patel DJ. Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30370-30379. doi: 10.1073/pnas.2018156117. Epub 2020 Nov 16. 10.1073/pnas.2018156117 PubMed 33199607
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