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Addgene

pEX-SP-YFP-STIM1(D76A)
(Plasmid #18859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Gateway destination
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Stromal interaction molecule 1
  • Alt name
    STIM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1995
  • Mutation
    D76A mutation - changed aspartate 76 to alanine. YFP is inserted between residues 1-23 (signal peptide) and residues 23-685 of STIM1. Lab plasmid MK2007.
  • GenBank ID
    NM_003156
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (unknown if destroyed)
  • 3′ cloning site attB2 (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEX-SP-YFP-STIM1(D76A) was a gift from Tobias Meyer (Addgene plasmid # 18859 ; http://n2t.net/addgene:18859 ; RRID:Addgene_18859)

  • For your References section:

    STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx. Liou J, Kim ML, Heo WD, Jones JT, Myers JW, Ferrell JE, Meyer T. Curr Biol. 2005 Jul 12. 15(13):1235-41. 10.1016/j.cub.2005.05.055 PubMed 16005298
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