E326K GBA pcDNA3.1
(Plasmid
#188581)
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PurposeEctopic expression E326K GBA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1 (+)
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Backbone manufacturerThermoFisher
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGBA
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SpeciesH. sapiens (human)
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Insert Size (bp)1750
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MutationE326K
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Entrez GeneGBA1 (a.k.a. GBA, GCB, GLUC)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (5', carrying its own ATG) (unknown if destroyed)
- 3′ cloning site XbaI (3', carrying its own TGA) (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E326K GBA pcDNA3.1 was a gift from Anthony Schapira (Addgene plasmid # 188581 ; http://n2t.net/addgene:188581 ; RRID:Addgene_188581) -
For your References section:
The GBA variant E326K is associated with alpha-synuclein aggregation and lipid droplet accumulation in human cell lines. Smith LJ, Bolsinger MM, Chau KY, Gegg ME, Schapira AHV. Hum Mol Genet. 2022 Sep 20:ddac233. doi: 10.1093/hmg/ddac233. 10.1093/hmg/ddac233 PubMed 36130205