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Addgene

Tet-off TauRD (P301L/V337M)
(Plasmid #188572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1-MAT2A (Addgene Plasmid #100521)
  • Total vector size (bp) 7920
  • Modifications to backbone
    pCW57.1-MAT2A plasmid was modified: MAT2A was replaced by TauRD and myc tag.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tau repeat domain
  • Alt name
    TauRD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    390
  • Mutation
    P301L and V337M
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Promoter pTight TRE
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-off TauRD (P301L/V337M) was a gift from Franz-Ulrich Hartl (Addgene plasmid # 188572 ; http://n2t.net/addgene:188572 ; RRID:Addgene_188572)

  • For your References section:

    The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system. Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Korner R, Holthusen H, Trinkaus VA, Dudanova I, Fernandez-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS. Nat Commun. 2023 Feb 2;14(1):560. doi: 10.1038/s41467-023-36058-2. 10.1038/s41467-023-36058-2 PubMed 36732333
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