pEX-SP-YFP-STIM1(23-685)
(Plasmid #18857)

• Depositing Lab: Tobias Meyer
• Publication: Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

Backbone

• Vector backbone: Gateway destination
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Stromal interaction molecule 1
• Alt name: STIM1
• Species: H. sapiens (human)
• Insert Size (bp): 1995
• Mutation: YFP is inserted between residues 1-23 (signal peptide) and residues 23-685 of STIM1. Lab plasmid MK2002.
• GenBank ID: NM_003156
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry