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pHAGE-EF1aL-W308Rmut_ABCA3-GFP
(Plasmid #188550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p1951_pHAGE-EFaL-GFP-W
  • Backbone size w/o insert (bp) 7036
  • Total vector size (bp) 12911
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ABCA3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5875
  • Mutation
    amino acid W308->R308 (ABCA3 gene)
  • GenBank ID
    NC_000016.10
  • Entrez Gene
    ABCA3 (a.k.a. ABC-C, ABC3, EST111653, LBM180, SMDP3)
  • Promoter EF1aL
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-EF1aL-W308Rmut_ABCA3-GFP was a gift from Darrell Kotton (Addgene plasmid # 188550 ; http://n2t.net/addgene:188550 ; RRID:Addgene_188550)

  • For your References section:

    Human pluripotent stem cell modeling of alveolar type 2 cell dysfunction caused by ABCA3 mutations. Sun YL, Hennessey EE, Heins H, Yang P, Villacorta-Martin C, Kwan J, Gopalan K, James M, Emili A, Cole FS, Wambach JA, Kotton DN. J Clin Invest. 2024 Jan 16;134(2):e164274. doi: 10.1172/JCI164274. 10.1172/JCI164274 PubMed 38226623
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