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pX330-U6-W308_guide-CBh-hSpCas9
(Plasmid #188546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSPCas9(BB)-2A-GFP
  • Backbone size w/o insert (bp) 8467
  • Total vector size (bp) 8487
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA
  • gRNA/shRNA sequence
    GAGGAAGAACAAGAGGAACC
  • Species
    H. sapiens (human)
  • Promoter U6
  • Tag / Fusion Protein
    • T2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-U6-W308_guide-CBh-hSpCas9 was a gift from Darrell Kotton (Addgene plasmid # 188546 ; http://n2t.net/addgene:188546 ; RRID:Addgene_188546)

  • For your References section:

    Human pluripotent stem cell modeling of alveolar type 2 cell dysfunction caused by ABCA3 mutations. Sun YL, Hennessey EE, Heins H, Yang P, Villacorta-Martin C, Kwan J, Gopalan K, James M, Emili A, Cole FS, Wambach JA, Kotton DN. J Clin Invest. 2024 Jan 16;134(2):e164274. doi: 10.1172/JCI164274. 10.1172/JCI164274 PubMed 38226623
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