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Addgene

pYPQ230-RV
(Plasmid #188544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Total vector size (bp) 6939
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible LbCas12a-RV entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbCas12a-RV
  • Species
    Lachnospiraceae bacterium ND2006
  • Mutation
    G146R, R182V
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • NLS (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ230-RV was a gift from Yiping Qi (Addgene plasmid # 188544 ; http://n2t.net/addgene:188544 ; RRID:Addgene_188544)

  • For your References section:

    Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants. Zhang L, Li G, Zhang Y, Cheng Y, Roberts N, Glenn SE, DeZwaan-McCabe D, Rube HT, Manthey J, Coleman G, Vakulskas CA, Qi Y. Genome Biol. 2023 Apr 30;24(1):102. doi: 10.1186/s13059-023-02929-6. 10.1186/s13059-023-02929-6 PubMed 37122009
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