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Addgene

phABCA3-eGFP_CRISPR_Donor
(Plasmid #188540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Total vector size (bp) 8145
  • Vector type
    CRISPR ; Donor for homologous recombination
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phABCA3-eGFP_CRISPR_Donor was a gift from Darrell Kotton (Addgene plasmid # 188540 ; http://n2t.net/addgene:188540 ; RRID:Addgene_188540)

  • For your References section:

    Heterogeneity in Human Induced Pluripotent Stem Cell-derived Alveolar Epithelial Type II Cells Revealed with ABCA3/SFTPC Reporters. Sun YL, Hurley K, Villacorta-Martin C, Huang J, Hinds A, Gopalan K, Caballero IS, Russo SJ, Kitzmiller JA, Whitsett JA, Beers MF, Kotton DN. Am J Respir Cell Mol Biol. 2021 Oct;65(4):442-460. doi: 10.1165/rcmb.2020-0259OC. 10.1165/rcmb.2020-0259OC PubMed 34101541
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