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pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE
(Plasmid #188539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOTTC1512 - pscAAV CMV-IE CDNFsigpep-EGFP
  • Backbone manufacturer
    NIDA GEVVC
  • Backbone size w/o insert (bp) 4388
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    secreted EGFP
  • Alt name
    secreted EGFP with ERS
  • Species
    Synthetic
  • Insert Size (bp)
    825
  • Promoter CMV-IE
  • Tags / Fusion Proteins
    • CDNF(1-28) (N terminal on insert)
    • THPKTEL WPRE (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV-Forward CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer WPRE R1 ATGAAAGCCATACGGGAAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE was a gift from Brandon Harvey (Addgene plasmid # 188539 ; http://n2t.net/addgene:188539 ; RRID:Addgene_188539)
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