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pSerOTS-C1* (V70)
(Plasmid #188537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKD-pSerRS-Ef-pSer
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pSerRS
  • Species
    Methanococcus maripaludis
  • Insert Size (bp)
    1620
  • Mutation
    mutations resulting from laboratory evolution
  • Promoter glnS*

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Ef-pSer
  • Species
    Methanococcus maripaludis
  • Insert Size (bp)
    1185
  • Mutation
    mutations resulting from laboratory evolution

Gene/Insert 3

  • Gene/Insert name
    tRNApSer
  • Species
    Methanococcus maripaludis
  • Insert Size (bp)
    220

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSerOTS-C1* (V70) was a gift from Jesse Rinehart (Addgene plasmid # 188537 ; http://n2t.net/addgene:188537 ; RRID:Addgene_188537)

  • For your References section:

    System-wide optimization of an orthogonal translation system with enhanced biological tolerance. Mohler K, Moen JM, Rogulina S, Rinehart J. Mol Syst Biol. 2023 Aug 8;19(8):e10591. doi: 10.15252/msb.202110591. Epub 2023 Jul 21. 10.15252/msb.202110591 PubMed 37477096
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