pAG416GPD-SEC7-Cerulean
(Plasmid
#18851)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAG416GPD
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Backbone manufacturerpRS416
- Backbone size w/o insert (bp) 7497
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSEC7
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)6029
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Entrez GeneSEC7 (a.k.a. YDR170C)
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Tag
/ Fusion Protein
- Cerulean (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site GATEWAY CLONING (not destroyed)
- 3′ cloning site GATEWAY CLONING (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG416GPD-SEC7-Cerulean was a gift from Susan Lindquist (Addgene plasmid # 18851 ; http://n2t.net/addgene:18851 ; RRID:Addgene_18851) -
For your References section:
The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis. Gitler AD, Bevis BJ, Shorter J, Strathearn KE, Hamamichi S, Su LJ, Caldwell KA, Caldwell GA, Rochet JC, McCaffery JM, Barlowe C, Lindquist S. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):145-50. 10.1073/pnas.0710685105 PubMed 18162536