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Addgene

SpRY dCas9 KRAB
(Plasmid #188501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPR v2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Streptococcus pyogenes
  • Mutation
    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Stbl3 is also a suitable growth strain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpRY dCas9 KRAB was a gift from Isaac Hilton (Addgene plasmid # 188501 ; http://n2t.net/addgene:188501 ; RRID:Addgene_188501)

  • For your References section:

    Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells. Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB. ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 10.1021/acssynbio.2c00156. Epub 2022 Sep 26. 10.1021/acssynbio.2c00156 PubMed 36162812
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