Cdk2-DN-HA
(Plasmid #1885)

• Depositing Lab: Sander van den Heuvel
• Publication: van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Backbone

• Vector backbone: pCMV-neo-Bam
• Backbone size w/o insert (bp): 6550
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cdk2
• Alt name: cyclin-dependent kinase 2
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: D146N, dominant negative
• Entrez Gene: CDK2 (a.k.a. CDKN2, p33(CDK2))
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Bglob-intron-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See scanned map.
Addgene's NGS results confirmed that the noted mutation is D145N compared to NP_001789.2.